ANTIBIOTIC SUSCEPTIBILITY PROFILE AND ITS CORRELATION TO BIOFILM FORMATION AND MANNOSE RESISTANT HEMAGGLUTINATION IN EXTRAINTESTINAL PATHOGENIC ESCHERICHIA COLI.

Abstract

The present study was conducted to gain an insight into drug resistance and virulence factors in extraintestinal pathogenic   Escherichia coli. This study was carried out on 150 Escherichia coli isolated from extraintestinal infections. Antimicrobial susceptibility testing was done by modified Kirby Bauer disc diffusion method. Extended spectrum β- lactamase (ESBL) production was confirmed by phenotypic confirmation tests. They were also screened for virulence factors, namely biofilm production, hemolysin, mannose resistant hemagglutination (MRHA), and gelatinase by standard methods. Majority of isolates included in this study were sensitive to imepenem (98%), amikacin (84.66%) and piperacillin-tazobactam (71.33%). In this study 76 isolates were ESBL producers (50.66%), but multi drug resistance was seen in many of isolates. Gelatinase activity was seen in 09 isolates (6%). Out of 150 isolates, 57 (38%) isolates were MRHA positive, 89 (59.33%) strains have formed biofilm and 43 (28.66%) isolates were   hemolysin producers.  The present study shows the expression of multiple virulence factors and multi drug resistance in extraintestinal pathogenic Escherichia coli. The present study also shows significant correlation between biofilm production, drug resistance and multiple virulence factors.