PHYSICOCHEMICAL, PRELIMINARY PHYTOCHEMICAL AND ANTIBACTERIAL INVESTIGATION ON LEAVES OF CARDIOSPERMUM CANESCENS WALL.

Abstract

Effect of ethanol, methanol and petroleum ether extracts from the leaves of
Cardiospermum canescens Wall. was studied against human pathogenic bacterial
strains of Escherichia coli, Salmonella typhi and Klebsiella pneumonia and the antibiotic
sensitivity of bacterial strains was also analyzed by using Bauer–Kirby method. Finally
leaf powder was subjected to physicochemical and preliminary phytochemical
evaluations to establish the quality and purity of the drug. In the Physicochemical
studies, loss on drying, total ash value, acid insoluble ash, water insoluble ash, various
extractive values etc., were carried out. Further, qualitative tests for various functional
groups like triterpenoids, alkaloids, glycosides etc., were carried out. HPTLC profile
was also established with ethyl acetate, and methanolic extracts. The results obtained
from the study concluded that leaf powder of this plant is a great potential source as an
antibacterial agent and preliminary phytochemical studies revealed the presence of
triterpenoids, alkaloids, tannins, flavonoids and proteins.