IMPLICATION OF CLAY MODIFIED ELECTRODE ON CYANIDE BIOSENSOR

Authors

  • CATHERINA BIJANG Chemistry Department, Mathematic and Natural Science Faculty, Pattimura University, Indonesia Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin University, Indonesia
  • ABD.WAHID WAHAB Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin University, Indonesia
  • MAMING Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin University, Indonesia
  • AHYAR AHMAD Chemistry Department, Mathematic and Natural Science Faculty, Hasanuddin University, Indonesia

Keywords:

Biosensor, Cyanide, Bentonite, Tyrosinase

Abstract

A novel, inexpensive and simple amperometric biosensor based on immobilization of tyrosinase enzyme onto bentonite and modified bentonite is applied for determination of cyanide.  The determination of cyanide was performed via its  inhibiting action on the tyrosinase electrode. Measurement was carried out using cathecol as  substrate. The enzymatically generated quinoid products were  electroreduced at 0.1 V vs Ag/AgCl. An extremely sensitive detection limit (2 x 10-7 M) was obtained for cyanide. Enzyme immobilitation onto an modified bentonite  seems to cause an increase in cyanide inhibition because of the greater surface area.

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Published

2014-03-31

How to Cite

CATHERINA BIJANG, ABD.WAHID WAHAB, MAMING, & AHYAR AHMAD. (2014). IMPLICATION OF CLAY MODIFIED ELECTRODE ON CYANIDE BIOSENSOR. International Journal of Pharma and Bio Sciences, 5(1), 101–107. Retrieved from https://ijpbs.in/index.php/journal/article/view/3020

Issue

Volume 5, Issue 1, 2014

Section

Research Articles

Categories

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