EFFECT OF FERMENTATION PARAMETERS AND VEGETABLE OILS ON PRODUCTION OF GAMMA LINOLENIC ACID BY MORTIERELLA RAMANNIANA.

Abstract

To optimize the production of gamma linolenic acid by Mortierella rammaniana  Methodology and Result: Effect of carbon source, nitrogen source, initial pH and oil supplementation was studied. The production of gamma linolenic acid reached 920 m gl^-1 when the production media were composed of 10% glucose, 1% peptone and 0.1% yeast extract pH 6.5 and incubated at 30^áµ’C for 144 hrs. Carbon and nitrogen sources showed major effect on the increase in gamma linolenic acid production. Repression in Δ 6-desaturase activity was observed in the presence of supplemented oil. This study highlights conditions for increasing gamma linolenic acid production by Mortierella rammaniana and an insight into rapidly gaining high production of polyunsaturated fatty acids.