ANTIBACTERIAL ACTIVITY OF PAPAIN HYDROLYSATES OF BUFFALO MILK WHEY PROTEIN AGAINST MASTITIS PATHOGENS

Abstract

Mastitis, a severe infectious disease among dairy cattle, is still a challenge for livestock treatment due to the increased infections by antibiotic resistant strains. Antimicrobial peptides could be the alternate for treating such drug resistant strains. In the present study, the whey proteins extracted from buffalo milk was subjected to enzymatic hydrolysis using trypsin, chymotrypsin, pepsin, papain and Proteinase K. Antibacterial assay carried out with these hydrolysates against Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus dysgalactiae isolated from mastitis milk showed that these hydrolysates have an inhibitory effect on their growth. Papain hydrolysates showed highest antibacterial activity against E.coli, Staphylococcus aureus, Streptococcus agalactiae and Streptococcus dysgalactiae. The fraction 4 (12 min fraction) of RP-HPLC purified papain hydrolysate showed highest antibacterial activity and that fraction has got Minimum inhibitory concentration of  100 µg/ml  against E.coli and 150 µg/ml against Staphylococcus aureus, 100 µg/ml  against Streptococcus agalactiae and 100 µg/ml  against Streptococcus dysgalactiae.