ANTIBIOTIC RESISTANCE AND MOLECULAR DNA FINGERPRINTING OF STAPHYLOCOCCUS AUREUS FROM CLINICAL SAMPLES

Abstract

Infections caused by antibiotic-resistant bacteria result in higher mortality and antibiotic resistance are a recognized major public health threat. S. aureus is a gram-positive microorganism with numerous virulence factors having the ability to acquire antibiotic resistance from the environment. The pathogenic S. aureus were isolated from hospital samples and indentified on the basis of morphological and biochemical characteristics. The antibiotic resistance pattern of these samples was determined by disc diffusion method against 10 antibiotics (Amoxicillin, Gentamicin, Methionine, Cloxacillin, Fluconazol, Ampicillin, Rifampcin, Tetracycline, Azithromycin and Cefixime). Furthermore, DNA fingerprint was performed for these samples using Random primers. Then dendrogram analysis was done to determine the genomic similarity of these samples.