RP-HPLC METHOD FOR THE QUANTIFICATION OF GEMCITABINEIN FORMULATIONS

Authors

  • SUBHASHINI.EDLA Dept of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar. GUNTUR
  • B.SYAMA SUNDHAR Dept of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar. GUNTUR

Keywords:

Gemcitabine, RP-HPLC, UV detection, recovery, precise, 282nm

Abstract

A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gemcitabine in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of Methanol: Acetonitrile: Water 80:15:5v/v. The UV detector wavelength was fixed at 282nm. In chromatogram the retention of Gemcitabine was 5.02min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for regular analysis of Gemcitabine in tablet dosage form.

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Published

2013-09-30

How to Cite

SUBHASHINI.EDLA, & B.SYAMA SUNDHAR. (2013). RP-HPLC METHOD FOR THE QUANTIFICATION OF GEMCITABINEIN FORMULATIONS. International Journal of Pharma and Bio Sciences, 4(3), 512–518. Retrieved from https://ijpbs.in/index.php/journal/article/view/2485

Issue

Volume 4, Issue 3, 2013

Section

Research Articles

Categories

License

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