EXPRESSION OF HUMAN ERYTHROPOIETIN GENE IN HELA CELLS

Authors

  • U. TIWARI Faculty of Biological Engineering, Shobhit University, Modipuram, Meerut, India
  • K. AGARWAL Faculty of Biological Engineering, Shobhit University, Modipuram, Meerut, India
  • A. RAI Institute of Biotechnology and IT, 197, Mudia Ahmadnagar, Bareilly, India
  • N. RAI Department of Biotechnology, Graphic Era University, Dehradun, Uttarakhand, India
  • P. PAL Faculty of Biological Engineering, Shobhit University, Modipuram, Meerut, India

Keywords:

HeLa cells, Erythropoietin gene, pSin Vector, Replicase Vector, Gene Expression.

Abstract

The cloned human erythropoietin (pSin.epo.hu) gene in pSin vector was transfected in HeLa cells. The protein expression was assayed by immuno assay, SDS-PAGE and western blotting. The cells were found to express the protein with development of purple color precipitate in Immunoperoxidase test. Intense purple coloration of cells was observed in HeLa cells transfected with pSin.epo.hu. The vector alone and untransfected healthy cells failed to show any coloration confirmed no expression of proteins. By SDS-PAGE and western blotting, the expressed erythropoietin (34 kDa) was confirmed.

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Published

2013-06-30

How to Cite

U. TIWARI, K. AGARWAL, A. RAI, N. RAI, & P. PAL. (2013). EXPRESSION OF HUMAN ERYTHROPOIETIN GENE IN HELA CELLS. International Journal of Pharma and Bio Sciences, 4(2), 1129–1137. Retrieved from https://ijpbs.in/index.php/journal/article/view/2403

Issue

Volume 4, Issue 2, 2013

Section

Research Articles

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