ANTIOXIDANT ACTIVITY AND ANALYSIS OF POLYPHENOLS FROM THE LEAF EXTRACTS OF CALOTROPIS PROCERA THROUGH ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

Authors

  • M. REMYA Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions University, Kancheepuram 603 104, India.
  • R. ANANTHAN National Institute of Nutrition and Health, Hyderabad, Andhra Pradesh, INDIA.
  • SUBHA SIVASANKAR Department of Biotechnology, Aarupadai Veedu Institute of Technology, Vinayaka Missions University, Kancheepuram 603 104, India.

Keywords:

Antioxidant activity, Calotropis procera, DPPH scavenging, reducing power, Inhibition of Nitric oxide radical, UPLC

Abstract

Antioxidant effects of the  Petroleum ether extracts of  the leaves  of Calotrois procera were tested on the basis of scavenging activity of the free radical  DPPH (1, 1-diphenyl-2-picryl-hydrazyl), reducing power and  inhibition of nitric oxide radical . The Petroleum Ether extracts of C .procera showed the highest antioxidant activity and the activity of the extract increased with increasing concentration. The total phenolic content of the leaf extract was found to be 38.23 µg pyrocatechol equivalents. Qualitative determination  of 10 polyphenols (Gallic acid, 3,4 Dihy droxy B-acid, Epi_GC Gallate, p-Coumaric acid, Ellagic acid, Quercetin, Apigenin, Hesperitin, Luteoline, Kaempherol) was made using UPLC .

Downloads

Published

2013-03-31

How to Cite

M. REMYA, R. ANANTHAN, & SUBHA SIVASANKAR. (2013). ANTIOXIDANT ACTIVITY AND ANALYSIS OF POLYPHENOLS FROM THE LEAF EXTRACTS OF CALOTROPIS PROCERA THROUGH ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Pharma and Bio Sciences, 4(1), 920–926. Retrieved from https://ijpbs.in/index.php/journal/article/view/2108

Issue

Volume 4, Issue 1, 2013

Section

Research Articles

Categories