EFFECT OF PIPER NIGRUM ON IN-VITRO RELEASE OF ISONIAZID FROM ORAL MICROSPHERES

Abstract

The purpose of the study was to prepare and optimize a sustained release formulation of isoniazid microspheres and to study the effect of Piper nigrum (Black Pepper) on its in-vitro release. Microspheres containing isoniazid were prepared using double emulsification and complex coacervation methods. The critical formulation variables were concentration of polymer, drug - polymer ratio, cross-linking agent concentration and cross-linking time. The time to release 85% of the contents of the microspheres (t₈₅) was used as the measure for the release time of the drug. The microspheres were optimized on the basis of their particle size, percentage yield, entrapment efficiency, bioadhesion study and in vitro drug release. The in-vitro release of the optimized batches of isoniazid microspheres was enhanced to the extent of 100-107%, by coadministration of 10 or 15 mg of bioenhancer.