DEVELOPMENT OF SOME NEW AND SENSITIVE ANALYTICAL METHOD FOR THE ESTIMATION AND VALIDATION OF LEVOFLOXACIN BY REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (RP-HPLC)

Abstract

A Rapid, precise, accurate, specific and simple RP-HPLC method was developed for determination of Levofloxacin in pharmaceutical formulation. The presented method is simple, since diluted samples are directly used without any preliminary chemical derivatisation or purification steps. A RP-HPLC assay utilized. A HPLC assay utilized symmetry C – 18 (4.6× 150mm, 5 mm), with mobile phase composition of Acetonitrile: Potassium dihydrogen orthophosphate [60:40] of pH 3 was used, and flow rate was 0.7 mL min^-1 with UV detection at 295 nm. The retention time Levofloxacin of was 2.448 min. The total HPLC run time was less than 5 min. Linearity was observed over concentration range of 20-60 µg/ml for Levofloxacin. The proposed method was validated for various ICH parameters like linearity, limit of detection, accuracy, precision, ruggedness, robustness, and system suitability. Commercial tablet formulations and laboratory prepared dilutions were successfully analyzed using the developed methods.