QUANTIFICATION OF ASPARTIC ACID BY HPTLC METHOD IN COCCINIA INDICA CRUDE DRUG, LAB EXTRACT AND COMMERCIAL EXTRACT

Abstract

The aim of the present study is to quantify the amount of aspartic acid in Coccinia indica crude drug, lab extract and commercial extract by HPTLC method. The stationary phase was precoated aluminium silica gel G F ₂₅₄ Plates. The mobile phase for aspartic  acid was  n-Butanol: Glacial acetic Acid:  Water (50: 10: 40). The methanolic solution of standard aspartic acid (1 mg/ ml concentration) 5 µl, crude drug methanolic solution 7 µl (100 mg/ ml concentration), lab extracts methanolic solution 7 µl (100 mg/ ml concentration) and commercial extracts methanolic solution 7 µl (100 mg/ ml concentration) were applied as 7mm babd width using CAMAG Linomat IV applicator. The mobile phase was n-Butenol: Glacial acetic Acid:  Water (50: 10: 40) and scanned by CAMAG Scanner III CATS (4.06) software at wavelength of 544 nm. The R_f of the sample Coccinia indica crude drug, lab extract and commercial extract were matching with the standard R_f   of marker compound aspartic acid. The amount of aspartic acid was calculated by comparing the peak area of standard and the same in the crude drug, lab extract and commercial extract. The content of aspartic acid was found to be 0.2778 % w/w, 0.9205 % w/w & 0.3057 % w/w for Coccinia indica crude drug, lab extract and commercial extract respectively. The calibration curve of standard aspartic acid was prepared by method of linear regression, using 1 mg/ml standard solution. The obtained data was subjected to statistical analysis using linear regression analysis and the correlation coeffiecient were determined. The correlation coeffiecient of aspartic acid was found to be 0.9899. In order to obtain precission and accuracy the recovery study were performed and result obtained with mean value 99.89 % for aspartic acid, which prove reproducibility of the result. The mean of % RSD value was found to be 1.54 for aspartic acid. This estimation technique is very much useful for the estimation of aspartic acid present in the various medicinal plants.