ISOLATION AND CHARACTERIZATION OF PHOSPHOLIPASE A2 FROM THE SPINES VENOM OF THE CROWN-OF-THORNS STARFISH ISOLATED FROM PAPUA ISLAND

Abstract

The crown of thorns starfish (COT) Acanthaster planci spine venom contains phospholipase A2 (PLA₂).  Recently, we have reported the rapid and efficient purification methods of it, especially for the PLA₂ from COT spine isolated in Ambon islands. The COT isolated in Papua islands has a different morphology compared with COT in Ambon islands, the spines were bigger and longer. To confirm the availability of PLA₂ in COT isolated in Papua, we isolated it by using our method. The activity of crude PLA₂ of it higher compare with crude PLA₂ isolated from COT spines from Ambon islands. Unfortunately, fractionated of ammonium sulfate at a concentration 20% is only slightly increased three times compared with the crude PLA₂. The result shows that the PLA₂ is calcium-dependent enzyme.