A COMPARATIVE STUDY OF NEUROPROTECTIVE POTENTIAL OF INHIBITOR OF PROLYL 4-HYDROXYLASE (PHD) IN RAT

Abstract

Cerebral stroke can decrease the blood supply to the brain at this condition stimulate the hypoxia inducible factor provide the neuroprotection in cerebral stroke. Hypoxia-inducible factor1 (HIF-1) is hetrodimer composed of HIF-1a and HIF-1b protein subunits. This transcription factor is essential for the activation of hypoxia inducible gene like erythropoietin, some glucose transporters, the glycolytic enzymes, and vascular endothelial growth factor. Because HIF-1 activation may promote cell survival in hypoxic tissues, we studied the effect of hypoxic preconditioning on HIF-1 expression in rat brain. To support the role of HIF-1 in protective preconditioning we also studied the effect of on other known HIF-1 inducer, cobalt chloride on HIF-1 expression and neuroprotection in rat brain. HIF-1a and HIF-1b protein levels were markedly increased after seven days chronic treatment of COCl₂ different dose (15mg/kg, 17.5mg/kg, 20mg/kg, 22.5mg/kg) and moderately increased the brain protection, respectively compared with that of vehicle injected littermate controls.