Evaluation Of Antimicrobial Activity And Phytochemical Screening Of Ethanolic Extract Of Greater Duckweed, Spirodela Polyrrhiza

Authors

  • BASANTA KUMAR DAS Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • DURGA PRASAD DAS Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • JYOTIRMAYEE PRADHAN Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • BARSHA PRIYADARSHINEE Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • IPSITA SAHU Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • PRAGYAN ROY Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India
  • BIBUDHENDRA KUMAR MISHRA Fish Health Management Division, Central Institute of Freshwater Aquaculture (CIFA), Kausalyagnaga, Bhubaneswar-751 002, Odisha, India

Keywords:

Antimicrobial activity, alkaloid, Candida albicans, Minimum Inhibitory Concentration (MIC), phytochemical, Staphylococcus aureus

Abstract

The phytochemical screening of the various extracts of Spirodela polyrrhiza revealed the presence of alkaloids, steroids, flavonoids and saponins. Fourteen fractions were elucidated from the ethanolic extract using the silica gel column chromatography. Antimicrobial activities of the ethanol and fractions of ethanolic extract of S. polyrrhiza against Gram-negative strains of Aeromonas hydrophila (AH1), Psedomonas putida (ATCC 49128), P. aeruginosa (ATCC 35072), P. fluorescens (PF1), Vibrio parahaemolyticus (ATCC 17802), V. Alginolyticus (ATCC 17749), E. coli (EC1) and Gram-positive strain of Staphylococcus aureus (ATCC 6538) and fungal pathogens, Candida albicans and Saprolegnia parasitica were done by disc diffusion methods showed that the extract possess broad spectrum of activity against the test organisms with diameter of zones of inhibition ranging from 9.0 - 16.0 mm. The minimum inhibitory concentration (MIC) of the extract against the test organisms were found to fall between 30 - 200 µg/ml.

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Published

2012-09-30

How to Cite

BASANTA KUMAR DAS, DURGA PRASAD DAS, JYOTIRMAYEE PRADHAN, BARSHA PRIYADARSHINEE, IPSITA SAHU, PRAGYAN ROY, & BIBUDHENDRA KUMAR MISHRA. (2012). Evaluation Of Antimicrobial Activity And Phytochemical Screening Of Ethanolic Extract Of Greater Duckweed, Spirodela Polyrrhiza. International Journal of Pharma and Bio Sciences, 3(3), 822–833. Retrieved from https://ijpbs.in/index.php/journal/article/view/1616

Issue

Volume 3, Issue 3, 2012

Section

Research Articles

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