Formulation Of High Laccases Production Media Using Ghee Residue Or Peptone As An Inducer By Trametes Hirsuta

Abstract

Trametes hirsuta (GenBank Accession Number: AB429065.1), a basidiomycete fungus was used for the production of laccases using different carbon sources namely glucose, sawdust, rice bran and wheat bran. Use of organic medium (agricultural/industrial wastes) is aimed to bring down the cost of media. Among these four carbon sources, the best laccase production was obtained in sawdust followed by wheat bran and rice bran. To improve laccases production, ghee residue (1%W/V), a byproduct of milk industry was added to the above media. For comparison, media with peptone (1% W/V) was used. The use of ghee residue in the formulation of the laccases production media by Trametes hirsuta has been found to be beneficial in laccases production. The production of laccases in presence of peptone and ghee residue was 85 folds and 7 folds more respectively than in the medium with only glucose. Similarly an increase of 11 folds and 9 folds in laccases production was obtained in the media containing wheat bran with peptone and ghee residue respectively as compared to the controls with only wheat bran. The production was found to be lowered in the medium with saw dust when peptone or ghee residue was added additionally. The production of laccases was found to be lowest in media containing only Glucose (0.44 U/ml). The optimum temperature was found to be 70⁰C and pH 5.0 for the laccases activity. The ghee residue and peptone seem to be acting as inducers in the production of laccases.